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Updated: Jun 22, 2026
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
Published on: January 30, 2017
Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1)
K H Chan1, S T Lai, L L M Poon
1Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Background:
A novel swine origin influenza virus (S-OIV) (H1N1) is spreading worldwide and threatens to become pandemic.
Objectives:
Determine analytical sensitivity of selected commercially available rapid influenza antigen detection tests in detecting S-OIV H1N1.
Study Design:
Serial dilutions of two S-OIV isolates, one seasonal influenza A (H1N1) isolate and a nasopharyngeal aspirate from a patient with S-OIV disease were tested in five commercially available influenza antigen detection tests and by virus isolation in cell culture. Viral M gene copy number was determined by quantitative PCR methods.
Results:
The analytical sensitivity of the five influenza antigen detection tests for S-OIV (tissue culture infectious dose 50 (TCID(50)) log(10)3.3-4.7 was comparable with that of seasonal influenza (TCID(50) log(10)4.0-4.5).
Conclusion:
The analytical sensitivity of the selected influenza A antigen detection tests for detection of S-IOV was comparable with that of seasonal influenza H1N1.
