Related Experiment Videos
A short synthetic adaptor as second-strand primer in the construction of cDNA libraries by the vector-primer method
E Boel1, A L Hjorth, K B Møller
1Molecular Genetics, Bioscience Novo Nordisk A/S, Bagsvoerd, Denmark.
Biotechniques
|July 1, 1991
Abstract:
The construction of high quality cDNA libraries is one of the most important yet technically challenging procedures in the study of gene structure and function. The present report presents a simplification of the classical Okayama-Berg protocol for the construction of plasmid libraries. The introduction of a short synthetic oligonucleotide as a second-strand adaptor facilitates the optimization of library construction and allows for quick adaptation of almost any vector as a cDNA cloning vehicle.