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Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Published on: June 23, 2018

Cyclodextrins promote protein aggregation posing risks for therapeutic applications.

Min S Wang¹, Shanta Boddapati, Michael R Sierks

¹Department of Chemical Engineering, Arizona State University, Tempe, AZ 85287-6006, USA.

Biochemical and Biophysical Research Communications

|June 23, 2009

Summary

Cyclodextrins alter beta-amyloid aggregation, potentially increasing toxicity in brain diseases like Alzheimer's. Caution is advised when using cyclodextrins for blood-brain barrier delivery or neurological treatments.

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Area of Science:

Neuroscience
Biochemistry
Pharmacology

Background:

Neurofibrillary tangles (NFTs) are key in neurodegenerative diseases like Alzheimer's (AD).
Elevated cholesterol is linked to NFTs, and cyclodextrins manage cholesterol in Niemann-Pick C disease models.
Cyclodextrins are explored as drug carriers across the blood-brain barrier (BBB).

Purpose of the Study:

To investigate the impact of cyclodextrins on beta-amyloid (Abeta) aggregation.
To assess how cyclodextrins affect the toxicity of Abeta, a protein implicated in AD.

Main Methods:

Studied the aggregation kinetics of Abeta in the presence of cyclodextrins.
Examined morphological changes in Abeta aggregates induced by cyclodextrins.
Assessed the toxicity of cyclodextrin-modified Abeta on SH-SY5Y neuroblastoma cells.

Main Results:

Cyclodextrins significantly altered Abeta aggregation rates.
Cyclodextrins induced morphological changes in Abeta aggregates.
These modified Abeta aggregates exhibited enhanced toxicity to neuronal cells.

Conclusions:

Cyclodextrins can promote the toxic aggregation of Abeta.
Careful consideration is needed when using cyclodextrins for BBB delivery or treating brain disorders due to potential Abeta-induced toxicity.