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Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes
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Pattern of tau isoforms expression during development in vivo.

Torsten Bullmann1, Max Holzer, Hiroshi Mori

  • 1Paul Flechsig Institute of Brain Research, Department of Cellular and Molecular Mechanisms of Neurodegeneration, University of Leipzig, Jahnallee 59, 04019 Leipzig, Germany.

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
|June 23, 2009
PubMed
Summary

The developmental shift in microtubule-associated protein tau (MAPT) isoforms in rat brain regions occurs similarly, coinciding with synapse formation and stable microtubule appearance, not neurogenesis.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Neuronal morphology and axonal growth are influenced by microtubule-associated protein tau (MAPT) isoforms.
  • MAPT plays a crucial role in microtubule stability and neuronal development.

Purpose of the Study:

  • To investigate the developmental expression patterns of MAPT isoforms in different rat brain regions.
  • To correlate MAPT isoform shifts with neurodevelopmental events like neurogenesis, synaptogenesis, and microtubule stabilization.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) to analyze MAPT mRNA expression.
  • Western blotting to quantify MAPT protein isoform levels.
  • Immunohistochemistry to assess cell-type specific MAPT expression and tubulin modifications indicative of microtubule stability.

Main Results:

  • The transition from fetal to adult MAPT isoforms occurred similarly in the cerebral cortex and cerebellum, despite their different developmental timelines.
  • Cell type-specific expression of MAPT isoforms was observed during development and in adulthood.
  • The shift in MAPT isoforms did not correlate with neurogenesis, migration, or volume growth but coincided with synapse formation and the emergence of stable microtubules.

Conclusions:

  • The developmental regulation of MAPT isoforms is conserved across different brain regions with distinct temporal development.
  • MAPT isoform dynamics are closely linked to the maturation of neuronal connectivity and microtubule stability rather than early developmental processes.