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Related Experiment Video

Updated: Jun 22, 2026

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
07:07

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

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Direct pulp capping with a self-etching adhesive system: histopathologic evaluation in dogs' teeth.

Lea A B da Silva1, Aldevina C de Freitas, Fabrício K de Carvalho

  • 1Department of Pediatric Clinics, Preventive and Community Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. lea@forp.usp.br

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
|June 23, 2009
PubMed
Summary

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Direct pulp capping with a self-etching adhesive system failed to promote pulp repair in dogs, showing no dentin bridge formation or signs of healing after 70 days. Calcium hydroxide, however, demonstrated successful histopathological outcomes.

Area of Science:

  • Endodontics
  • Dental Materials Science
  • Histopathology

Background:

  • Direct pulp capping aims to preserve pulp vitality and promote healing.
  • Traditional materials like calcium hydroxide are known for their regenerative potential.
  • Novel adhesive systems are being explored for direct pulp capping, but their efficacy requires thorough evaluation.

Purpose of the Study:

  • To histopathologically evaluate the response of dental pulp and periradicular tissues after direct pulp capping using an all-in-one self-etching adhesive system in a canine model.
  • To compare the outcomes with established materials like calcium hydroxide and zinc oxide-eugenol.

Main Methods:

  • Forty teeth from four dogs were divided into three groups: self-etching adhesive system (n=20), calcium hydroxide (n=10), and zinc oxide-eugenol (n=10).

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Last Updated: Jun 22, 2026

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
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  • Teeth were histologically analyzed at 7 and 70 days post-procedure.
  • Histological assessment focused on dentin bridge formation, inflammatory response, and tissue repair.

    • Main Results:

    • At 7 days, no dentin bridge was observed in the self-etching adhesive or zinc oxide-eugenol groups, which showed pulpal inflammation. Calcium hydroxide showed only mild inflammation.
    • At 70 days, neither the self-etching adhesive nor zinc oxide-eugenol groups formed dentin bridges; severe inflammation and necrosis were present.
    • All calcium hydroxide specimens demonstrated dentin bridge formation with no inflammation or resorption, and no bacteria were detected in any group.

    Conclusions:

    • Direct pulp capping with the evaluated self-etching adhesive system resulted in a 100% histopathological failure rate.
    • The self-etching adhesive system did not facilitate pulp tissue repair or dentin bridge formation.
    • Calcium hydroxide remains a predictable material for direct pulp capping, promoting successful tissue healing.