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Assay conditions and validation of a new UV spectrophotometric method using microplates for the determination of

Iñigo Uriarte Pueyo1, María Isabel Calvo

  • 1Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Navarra, Pamplona (Navarra), Spain. iuripue@yahoo.com

Fitoterapia
|June 23, 2009
PubMed

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...