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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
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Increasing the coverage of a metapopulation consensus genome by iterative read mapping and assembly

Bas E Dutilh1, Martijn A Huynen, Marc Strous

  • 1Center for Molecular and Biomolecular Informatics, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Medical Center, Geert Grooteplein 28, 6525 GA, Nijmegen, The Netherlands, Germany. dutilh@cmbi.ru.nl

Bioinformatics (Oxford, England)
|June 23, 2009
PubMed
Summary

This study presents a novel method for assembling microbial genomes from complex populations using a reference genome scaffold. The technique improves genome completeness and accuracy, even with diverse strains present.

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