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Related Experiment Video

Updated: Jun 22, 2026

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
08:10

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

Published on: November 28, 2018

Simultaneous detection of many T-cell specificities using combinatorial tetramer staining.

Evan W Newell1, Lawrence O Klein, Wong Yu

  • 1Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.

Nature Methods
|June 23, 2009
PubMed
Summary

This study introduces a novel method for detecting numerous antigen-specific T cells simultaneously. The technique uses combinations of fluorescent peptide-major histocompatibility complex (pMHC) tetramers to identify over 15 T-cell specificities in one sample.

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Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

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Related Experiment Videos

Last Updated: Jun 22, 2026

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
08:10

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

Published on: November 28, 2018

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
15:42

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

Published on: March 6, 2009

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
13:58

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Published on: October 22, 2012

Area of Science:

  • Immunology
  • Cellular immunology
  • T-cell biology

Background:

  • Direct detection of antigen-specific T cells is crucial in immunology.
  • Current methods using peptide-major histocompatibility complex (pMHC) tetramers are limited in the number of specificities that can be assessed simultaneously.
  • This limitation hinders comprehensive T-cell analysis.

Purpose of the Study:

  • To develop and validate a method for simultaneous detection and enrichment of a large number of T-cell specificities.
  • To overcome the limitation of assessing multiple T-cell specificities in a single sample.
  • To advance T-cell analysis in basic and clinical immunology.

Main Methods:

  • Utilized combinations of fluorescently labeled peptide-major histocompatibility complex (pMHC) tetramers.
  • Applied the method to human blood samples.
  • Validated the simultaneous detection and enrichment of multiple T-cell specificities.

Main Results:

  • Successfully detected and enriched for more than 15 T-cell specificities concurrently.
  • Demonstrated the feasibility of multiplexed T-cell specificity analysis.
  • Validated the method's effectiveness in a single human blood sample.

Conclusions:

  • The developed method significantly expands the capacity for simultaneous T-cell specificity analysis.
  • This advancement facilitates more comprehensive T-cell profiling in immunological studies.
  • The technique offers a powerful tool for both basic research and clinical applications in immunology.