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Electrospray Ionization (ESI) Mass Spectrometry

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

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Related Experiment Video

Updated: Jun 22, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Electrospray mass spectrometry for quantitative plasma proteome analysis.

Hong Wang¹, Sam Hanash

¹Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 23, 2009

Summary

Electrospray ionization mass spectrometry (ESI-MS) enables soft ionization of large biomolecules. This chapter details a method for quantitative plasma protein profiling to discover cancer biomarkers.

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A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

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Published on: April 24, 2020

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Last Updated: Jun 22, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Published on: April 24, 2020

Area of Science:

Biochemistry
Analytical Chemistry
Proteomics

Background:

Electrospray ionization mass spectrometry (ESI-MS) is a key technique for analyzing large biomolecules.
Generating charged molecules for mass-to-charge ratio (m/z) analysis via ESI-MS requires a delicate process.
Quantitative profiling of plasma proteins is crucial for biomarker discovery, particularly in cancer research.

Purpose of the Study:

To present the mechanism of electrospray ionization.
To describe an experimental pipeline for quantitative profiling of plasma proteins using ESI-MS.
To highlight the application of this pipeline in cancer biomarker discovery.

Main Methods:

Brief presentation of the electrospray ionization mechanism.
Detailed description of a quantitative profiling pipeline: prefractionation immunodepletion, protein isotope tagging, 2D-HPLC separation of intact proteins, and LC-MS.
Application of the pipeline in cancer biomarker discovery studies.

Main Results:

The presented pipeline enables efficient quantitative profiling of plasma proteins.
The methodology is suitable for identifying potential cancer biomarkers.
ESI-MS provides a robust platform for complex proteomic analyses.

Conclusions:

The described experimental pipeline is effective for quantitative plasma protein profiling.
This approach aids in the discovery of novel cancer biomarkers.
ESI-MS is a powerful tool for advancing proteomic research and clinical applications.