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Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
Published on: May 20, 2020
[Application of a simple method for the detection of measles virus genome by loop-mediated isothermal amplification
Jian-hui Zhou1, Xiang Hou, Chao Chen
1Jilin Provincial Center for Disease Control and Prevention, Changchun 130062, China.
Objective:
A new simple RT-LAMP method was applied to detect measles virus nucleic acid and compared with nest-RT-PCR.
Methods:
Compare the detection rate of the RT-LAMP method with that of nest-RT-PCR by detecting measles virus nucleic acid from measles virus and clinical samples.
Results:
The nucleic acid positive rates of all 23 strains of measles virus are all 100% by the two methods. But to the detection of 18 clinical samples which are negative in measles isolation, the nest-RT-LAMP showed 56.52% positive rate of nucleic acid of measles virus and nest-RT-PCR showed 47.83%.
Conclusion:
RT-LAMP is more sensitive than nest-RT-PCR.
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