Related Experiment Video
Updated: Jun 22, 2026
An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay
Published on: January 26, 2019
[Research on an improved Lowry method to determine content of protein in Sabin IPV]
Wei Cai1, Ming-bo Sun, Hong-bo Chen
1Institute of Medical Biology, Chinese Academy of Medical Sciences, Pekin Union Medical College, Kunming 650118, China.
Objective:
To establish a method for the content determination of protein in Sabin IPV.
Methods:
Using lowry method combined with being precipitated by trichloroacetic acid to determine the content of protein in Sabin IPV. Changing different conditions to optimize the experiment to establish a improved lowry method. And the sample recovery test was also conducted.
Results:
The method can exclude the interference of free aminoacid, phenols and some other additives. The calibration curve was in good linearity of protein within the range of 2.5 microg/ml-40 Microg/ml, r = 0.9998. Under the best conditions, the mean recovery was 95.32%, the CV in a batch and between batches were both < 10%.
Conclusion:
The method can be used to determine the micro content of protein in vaccines.
More Related Videos
08:14Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
Published on: February 14, 2013
11:06Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts
Published on: August 8, 2016