Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 22, 2026

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
13:46

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

Published on: July 11, 2020

[MPP+ decreased BDNF expression in PC12 cells].

Yu-he Yuan1, Jian-dong Sun, Jin-feng Hu

  • 1Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

Yao Xue Xue Bao = Acta Pharmaceutica Sinica
|June 24, 2009
PubMed
Summary

1-methyl-4-phenylpyridinium (MPP+) reduces PC12 cell viability and brain-derived neurotrophic factor (BDNF) levels by inhibiting the MAPK/ERK pathway and CREB phosphorylation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A highly sensitive and simultaneous bifunctional fluorescent probe for detecting mitochondrial viscosity and nitroreductase activity in vivo.

Analytica chimica acta·2026
Same author

Alcimaphenols A and B, oligomeric denudaquinols with antiproliferative and antimigratory activities from the endangered plant Alcimandra cathcartii.

Bioorganic chemistry·2026
Same author

Corrigendum to 'Deletion in exon 5 of the SNCA gene and exposure to rotenone leads to oligomerization of α-synuclein and toxicity to PC12 cells' [Brain Research Bulletin 90 (2013) 127-131].

Brain research bulletin·2026
Same author

Targeted stabilization of astrocytic Cx43 gap junctions reverses depression-like behaviors via dual restoration of syncytial networks and glutamatergic homeostasis.

Neurochemistry international·2026
Same author

Targeted isolation of previously undescribed fortunefuroic acid-type triterpenoids from Keteleeria pubescens using <sup>1</sup>H NMR/LC-PDA-MS and assessment of ACL Inhibition.

Phytochemistry·2026
Same author

IMM-H004 attenuates neutrophil pyroptosis following ischemic stroke via a CKLF1 dependent mechanism.

International immunopharmacology·2026

Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Context:

  • 1-methyl-4-phenylpyridinium (MPP+) is a neurotoxin implicated in Parkinson's disease research.
  • PC12 cells are a widely used cell line for studying neuronal function and toxicity.
  • Understanding the molecular mechanisms of neurotoxicity is crucial for developing therapeutic strategies.

Purpose:

  • To elucidate the neurotoxic effects of MPP+ on PC12 cells.
  • To investigate the underlying molecular mechanisms, including effects on cell viability, protein levels, and signaling pathways.

Summary:

  • MPP+ significantly decreased PC12 cell viability in a dose-dependent manner.
  • MPP+ reduced brain-derived neurotrophic factor (BDNF) protein levels.
  • MPP+ inhibited the phosphorylation of ERK and the phosphorylation/transactivation of CREB, a key transcription factor for BDNF, via the MAPK/ERK pathway, without affecting CaMK II activity.

More Related Videos

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells
05:49

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells

Published on: April 13, 2018

Related Experiment Videos

Last Updated: Jun 22, 2026

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
13:46

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

Published on: July 11, 2020

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells
05:49

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells

Published on: April 13, 2018

Impact:

  • Identifies the MAPK/ERK signaling pathway and CREB as critical mediators in MPP+-induced neurotoxicity.
  • Provides insights into the molecular basis of BDNF downregulation by MPP+.
  • Contributes to the understanding of neurodegenerative processes and potential therapeutic targets.