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Related Experiment Video

Updated: Jun 22, 2026

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Published on: July 23, 2016

siRNA delivery using peptide transduction domains.

Akiko Eguchi¹, Steven F Dowdy

¹Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, UCSD School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093-0686, USA.

Trends in Pharmacological Sciences

|June 24, 2009

Summary

Peptide transduction domains (PTDs) can effectively deliver short interfering RNAs (siRNAs) into cells, overcoming delivery barriers for potential siRNA-based therapies.

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Published on: March 25, 2015

Area of Science:

Biotechnology
Molecular Biology
Drug Delivery

Background:

Short interfering RNAs (siRNAs) show therapeutic promise but face cellular delivery challenges due to their size and charge.
Existing siRNA delivery systems require enhancement for improved cellular uptake.
Peptide transduction domains (PTDs) are known for their ability to cross cell membranes independently.

Purpose of the Study:

To review the use of PTDs for in vitro and in vivo siRNA delivery.
To discuss the potential of PTD-mediated siRNA delivery in therapeutic applications.

Main Methods:

Review of existing literature on PTDs and siRNA delivery.
Analysis of in vitro and in vivo studies utilizing PTD-siRNA conjugates.
Discussion of PTD mechanisms for cellular membrane translocation.

Main Results:

PTDs facilitate the cellular entry of siRNAs, overcoming natural membrane barriers.
PTDs have been successfully employed for delivering various molecules, including nucleic acids.
Studies demonstrate the in vitro and in vivo applicability of PTD-mediated siRNA delivery.

Conclusions:

PTDs represent a promising strategy for enhancing siRNA delivery and cellular uptake.
PTD-based systems offer potential for advancing siRNA-based therapeutic strategies.
Further research into PTD-siRNA systems could lead to novel disease treatments.