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Related Experiment Video

Updated: Jun 22, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Single-cell trapping utilizing negative dielectrophoretic quadrupole and microwell electrodes.

Ling-Sheng Jang¹, Pao-Hua Huang, Kung-Chieh Lan

¹Department of Electrical Engineering and Center for Micro/Nano Science and Technology, National Cheng Kung University, 1 University Road, Tainan 701, Taiwan. lsjang@ee.ncku.edu.tw

Biosensors & Bioelectronics

|June 24, 2009

Summary

This study introduces a microfluidic device for precise single-cell manipulation. It combines alternating current electrothermal (ACET) flow and negative dielectrophoretic (nDEP) traps for guiding and capturing cells.

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A Microfluidic-based Hydrodynamic Trap for Single Particles

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Area of Science:

Biophysics
Microfluidics
Cell Engineering

Background:

Individual cell handling is crucial for cell engineering applications like gene introduction and drug delivery.
Alternating current (AC) electrokinetics offers potential for microfluidic particle manipulation, including pumping, mixing, and concentration.
Non-uniform electric fields induce Joule heating and dielectrophoresis (DEP), influencing particle motion directly and indirectly via fluid flow.

Purpose of the Study:

To present a microfluidic device for specific guidance and capture of single particles and cells.
To utilize alternating current electrothermal (ACET) induced fluid flow and negative dielectrophoretic (nDEP) traps for cell manipulation.
To validate the device's performance in high-conductivity media relevant to biochemical and environmental applications.

Main Methods:

Development of a microfluidic device integrating AC electrokinetics.
Application of high frequencies (5-12 MHz) and high conductivity (1.25 S/m) culture medium.
Numerical modeling of particle motion influenced by ACET-induced fluid flow and nDEP forces.
Experimental validation of guided particle and cell manipulation.

Main Results:

The microfluidic device successfully guided and captured single particles and cells.
ACET-induced fluid flow effectively directed particles towards the nDEP trap.
Numerical simulations showed good agreement with experimental observations of particle motion.
The system demonstrated effectiveness in high-conductivity media, relevant for biochemical and environmental monitoring.

Conclusions:

The developed microfluidic device enables precise single-cell manipulation through a combination of ACET flow and nDEP trapping.
This technology holds promise for advanced cell engineering, biochemical analysis, and environmental monitoring.
The study validates the synergistic use of ACET and nDEP for controlled particle and cell manipulation in microfluidic systems.