Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strategies for Identifying Molecules of Interest in Large Chemical Spaces.

Journal of chemical information and modeling·2026
Same author

Local autophagy impairment triggers brain-wide presynaptic remodeling and resilience.

The EMBO journal·2026
Same author

Reply to "Correspondence: Androgen receptor mRNA in glioblastoma: a reliable marker of tumor burden or a statistical artefact?"

Molecular biology reports·2026
Same author

Disentangling direct and indirect genetic pathways to neurodevelopmental risk: brain structure and behavior in a population-based parent-offspring trio study.

Journal of child psychology and psychiatry, and allied disciplines·2026
Same author

Paediatric DNA methylation profile scores: a systematic review and open-source atlas.

EBioMedicine·2026
Same author

PASTA-4-PHT: a pipeline for automated security and technical audits for the personal health train.

BMC medical informatics and decision making·2026

Related Experiment Video

Updated: Jun 22, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Imaging interferometric microscopy-approaching the linear systems limits of optical resolution.

Yuliya Kuznetsova, Alexander Neumann, S R Brueck

    Optics Express
    |June 24, 2009
    PubMed
    Summary

    This study presents a synthetic aperture microscopy technique to overcome conventional optical resolution limits. The method achieves sub-wavelength resolution, approaching the theoretical lambda/4 limit for linear systems.

    More Related Videos

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

    Published on: December 1, 2016

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    Related Experiment Videos

    Last Updated: Jun 22, 2026

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

    Published on: December 1, 2016

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    Area of Science:

    • Optical microscopy
    • Nanotechnology
    • Image processing

    Background:

    • Conventional microscopy is limited by the Rayleigh criterion (~0.6*lambda/NA).
    • The theoretical optical resolution limit for linear systems is lambda/4 pitch.
    • Achieving lambda/4 resolution is challenging with standard microscopy.

    Purpose of the Study:

    • To present a synthetic aperture approach for enhanced optical resolution.
    • To extend previous developments in imaging interferometric microscopy.
    • To demonstrate sub-wavelength resolution beyond conventional limits.

    Main Methods:

    • Utilizing a synthetic aperture technique.
    • Extending imaging interferometric microscopy principles.
    • Employing a low numerical aperture (NA=0.4) optical system.

    Main Results:

    • Demonstrated resolution of 180-nm non-periodic features (lambda/3.52) with 633-nm illumination.
    • Achieved resolution of a 170-nm grating (lambda/3.72).
    • Maintained advantages of low-NA systems, including working distance and field-of-view.

    Conclusions:

    • The synthetic aperture method approaches the ultimate lambda/4 resolution limit.
    • This technique offers superior resolution without compromising practical microscopy parameters.
    • Enables high-resolution imaging with accessible optical systems.