Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to the...
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rotational excitation of molecules in the regime of strong ro-vibrational coupling: a comparison between an optical centrifuge and a transform-limited pulse.

Physical chemistry chemical physics : PCCP·2026
Same author

Arotinoid trometamol inhibits arsenic trioxide-stimulated keratinocyte proliferation via the Wnt, Shh, and bone morphogenetic protein signaling pathways.

Journal of biological regulators and homeostatic agents·2019
Same author

[Follow-up analysis on change of serum total cholesterol concentration in rural residents in Shanxi province].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2019
Same author

Raman spectroscopy of stored red blood cell concentrate within sealed transfusion blood bags.

The Analyst·2018
Same author

[Association between blood pressure related dietary patterns and identified cognitive performance in the elderly Chinese-a study by reduced rank regression method].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2018
Same author

[Characterization analysis of gM, gL genes of varicella zoster virus in six provinces of China].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2018

Related Experiment Video

Updated: Jun 22, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

Pulse optimization for Raman spectroscopy with cross-correlation frequency resolved optical gating.

S O Konorov, X G Xu, R F Turner

    Optics Express
    |June 24, 2009
    PubMed
    Summary
    This summary is machine-generated.

    Femtosecond pulse shaping enhances molecular vibration characterization by improving amplitude and phase detection accuracy. This advanced technique increases the reliability of spectral and temporal response measurements.

    More Related Videos

    Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
    09:57

    Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

    Published on: February 10, 2020

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
    09:57

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

    Published on: July 25, 2022

    Related Experiment Videos

    Last Updated: Jun 22, 2026

    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
    15:04

    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

    Published on: May 18, 2011

    Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
    09:57

    Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

    Published on: February 10, 2020

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
    09:57

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

    Published on: July 25, 2022

    Area of Science:

    • Physical Chemistry
    • Spectroscopy
    • Molecular Dynamics

    Background:

    • Accurate characterization of molecular vibrations is crucial for understanding chemical reactions and material properties.
    • Existing methods for detecting laser-induced vibrational coherence require high resolution for both amplitude and phase information.
    • Recent advancements have introduced methods for complete characterization, but performance can be limited.

    Purpose of the Study:

    • To improve the performance of complete molecular vibration characterization.
    • To enhance the accuracy and robustness of detecting laser-induced vibrational coherence.
    • To leverage femtosecond pulse shaping for advanced spectroscopic analysis.

    Main Methods:

    • Employing femtosecond pulse shaping to precisely control laser pulses.
    • Utilizing cross-correlation frequency resolved optical gating (XFROG) of Raman modes.
    • Generating rich interference patterns in two-dimensional spectrograms of coherent anti-Stokes Raman scattering (CARS).

    Main Results:

    • Demonstrated enhanced accuracy in retrieving spectral and temporal response of molecular vibrations.
    • Increased robustness of the characterization method against experimental noise.
    • Successful high-resolution detection of both amplitude and phase of vibrational coherence.

    Conclusions:

    • Femtosecond pulse shaping significantly improves the complete characterization of molecular vibrations.
    • The enhanced method offers greater precision and reliability for spectroscopic studies.
    • This technique provides a powerful tool for probing molecular dynamics with unprecedented detail.