Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Definitive correction for nonlinear and random phase-step detuning using the universal phase-shifting algorithm.

Applied optics·2026
Same author

Theoretical and experimental study of optical diffractometry based on Fresnel diffraction from a transmission phase step.

Applied optics·2023
Same author

Wavefront reconstruction of discontinuous phase objects from optical deflectometry.

Applied optics·2023
Same author

Wavelength-stepping algorithm for testing the thickness and front and back surfaces of optical plates with high signal-to-noise ratio.

Journal of the Optical Society of America. A, Optics, image science, and vision·2023
Same author

Simultaneous phase and amplitude modulation for dual-sensitivity profilometry of discontinuous objects.

Journal of the Optical Society of America. A, Optics, image science, and vision·2022
Same author

Moire profilometry through simultaneous dual fringe projection for accurate phase demodulation: a comparative study.

Applied optics·2021
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 22, 2026

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
12:14

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

Published on: August 12, 2013

Heterodyne two beam Gaussian microscope interferometer.

J Mauricio Flores, Moisés Cywiak, Manuel Servín

    Optics Express
    |June 24, 2009
    PubMed
    Summary
    This summary is machine-generated.

    We developed a new microscope interferometric technique using two Gaussian beams to measure microscopic optical surface roughness. This method provides a linear response, accurately measuring surface height with a high signal-to-noise ratio.

    More Related Videos

    Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy
    08:49

    Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

    Published on: December 1, 2023

    A Multimodal Wide-Field Fourier-Transform Raman Microscope
    06:48

    A Multimodal Wide-Field Fourier-Transform Raman Microscope

    Published on: December 30, 2025

    Related Experiment Videos

    Last Updated: Jun 22, 2026

    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
    12:14

    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

    Published on: August 12, 2013

    Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy
    08:49

    Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

    Published on: December 1, 2023

    A Multimodal Wide-Field Fourier-Transform Raman Microscope
    06:48

    A Multimodal Wide-Field Fourier-Transform Raman Microscope

    Published on: December 30, 2025

    Area of Science:

    • Optics
    • Metrology
    • Surface Science

    Background:

    • Accurate measurement of microscopic surface roughness is crucial for optical component performance.
    • Existing techniques may lack sensitivity or resolution for certain applications.

    Purpose of the Study:

    • To introduce a novel heterodyne interferometric microscope technique for measuring optical surface roughness at microscopic scales.
    • To demonstrate the linear response and high signal-to-noise ratio of the developed system.

    Main Methods:

    • Utilized a heterodyne interferometry setup with two Gaussian beams.
    • One beam acted as a probe, focused and reflected by the test surface.
    • A Bragg cell generated a modulating signal, enabling coherent superposition and detection.

    Main Results:

    • Analytical derivation confirmed linear system response under specific working conditions.
    • Probe beam size and photodetector signal amplitude were found to be proportional to surface height.
    • Experimental validation was performed on a blazed-reflecting grating sample.

    Conclusions:

    • The developed microscope interferometric technique offers a precise method for microscopic optical surface roughness measurement.
    • The system's linear response and high signal-to-noise ratio make it suitable for detailed surface topography analysis.