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Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
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Related Experiment Video

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Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
12:27

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Published on: November 25, 2009

Offset-apertured near-field scanning optical microscope probes.

M C Quong, A Y Elezzabi

    Optics Express
    |June 24, 2009
    PubMed
    Summary

    Near-field scanning optical microscope (NSOM) probes with offset apertures and metal-coated tips efficiently couple surface plasmons. This design enhances light intensity for improved optical probing resolution and throughput.

    Area of Science:

    • Optics and Photonics
    • Nanotechnology
    • Microscopy

    Background:

    • Near-field scanning optical microscopy (NSOM) is crucial for high-resolution imaging.
    • Traditional NSOM probes face limitations in light throughput and resolution.
    • Surface plasmon coupling offers potential for enhanced optical probe performance.

    Purpose of the Study:

    • To investigate novel NSOM probe designs for improved optical performance.
    • To analyze the optical field distribution and intensity at the probe apex.
    • To compare the throughput of offset-apertured probes with conventional designs.

    Main Methods:

    • Finite-difference time-domain (FDTD) calculations were employed to simulate probe designs.
    • Investigated probes featured subwavelength apertures offset from metallic or metal-coated dielectric tips.

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  • Analyzed the coupling of surface plasmons to the probe apex.

    • Main Results:

    • Offset apertures and metal-coated tips effectively couple surface plasmons to the probe apex.
    • A single-lobed probing optical spot with a full-width half maximum (FWHM) comparable to the apex diameter was achieved.
    • The designed probes demonstrated significantly higher throughput light intensities compared to conventional apertured NSOM.

    Conclusions:

    • Offset-apertured NSOM probes utilizing surface plasmon coupling offer enhanced light intensity.
    • This design provides a promising pathway for higher throughput and improved resolution in near-field microscopy.
    • The findings suggest a significant advancement in NSOM probe technology.