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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: Jun 22, 2026

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
06:53

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

Published on: May 17, 2020

Multifocal multiphoton microscopy (MMM) at a frame rate beyond 600 Hz.

Karsten Bahlmann, Peter T So, Michael Kirber

    Optics Express
    |June 24, 2009
    PubMed
    Summary
    This summary is machine-generated.

    This study presents a novel multifocal multiphoton microscope (MMM) for rapid 3D fluorescence imaging. The system achieves high-speed imaging of biological samples, enabling detailed observation of cellular processes like calcium waves.

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    Last Updated: Jun 22, 2026

    A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
    06:53

    A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

    Published on: May 17, 2020

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    Area of Science:

    • Biophysics
    • Optical Microscopy
    • Cellular Imaging

    Background:

    • High-speed three-dimensional (3D) fluorescence imaging is crucial for observing dynamic biological processes.
    • Existing microscopy techniques often face limitations in speed and resolution, hindering real-time cellular analysis.

    Purpose of the Study:

    • To develop and demonstrate a novel multiphoton microscope system capable of high-speed 3D fluorescence imaging.
    • To enhance imaging speed through parallel illumination and detection.

    Main Methods:

    • The system integrates a multifocal multiphoton microscope (MMM) with parallel illumination using a microlens array generating 36 foci.
    • Parallel detection is achieved using a custom-made, segmented charge-coupled device (CCD) camera operating at 1428 Hz with low read noise.
    • Foci scanning is performed using a resonance scanner.

    Main Results:

    • The multifocal multiphoton microscope (MMM) system achieves an imaging frame rate of 640 Hz.
    • Demonstrated high-speed imaging of fluorescent specimens, including calcium waves in fluo3-labeled cardiac myocytes.
    • Successfully captured spontaneous cell contractions in a 0.625-second movie sequence.

    Conclusions:

    • The developed multifocal multiphoton microscope (MMM) significantly advances high-speed 3D fluorescence imaging capabilities.
    • The parallel illumination and detection strategy enables unprecedented imaging speeds for dynamic biological studies.
    • This technology provides a powerful tool for investigating rapid cellular events in real-time.