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Related Experiment Video

Updated: Jun 22, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
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High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

[Not Available].

Jana Rolff1, Cornelia Dorn, Johannes Merk

  • 1Experimental Pharmacology & Oncology GmbH, Robert-Rössle-Street 10, 13122 Berlin, Germany.

Journal of Oncology
|June 24, 2009
PubMed
Summary

Multidrug resistance (MDR) in non-small cell lung cancer (NSCLC) is not strongly linked to common resistance markers. Protein and mRNA levels of MDR markers showed little correlation with drug response in patient-derived xenografts.

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Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Multidrug resistance (MDR) is a significant challenge in non-small cell lung cancer (NSCLC) treatment.
  • Previous studies on MDR markers in NSCLC have yielded conflicting results regarding their impact on chemosensitivity.

Purpose of the Study:

  • To investigate the correlation between MDR-related protein and mRNA expression and chemosensitivity in patient-derived NSCLC xenografts.
  • To analyze potential regulatory mechanisms of MDR markers following therapeutic interventions.

Main Methods:

  • Utilized 26 well-characterized patient-derived NSCLC xenografts.
  • Assessed chemosensitivity to etoposide, carboplatin, gemcitabine, paclitaxel, and erlotinib.
  • Quantified protein and mRNA expression of BCRP, LRP, MDR1, and MRP1.
  • Performed short-term treatment on four xenografts to study regulatory mechanisms.

Main Results:

  • A borderline correlation was observed between BCRP mRNA expression and etoposide response.
  • No significant correlation was found between constitutive mRNA/protein levels of investigated MDR markers and drug response.
  • Short-term therapeutic interventions did not significantly influence the expression levels of these MDR markers.

Conclusions:

  • The expression levels of investigated MDR proteins and mRNA do not appear to play a major role in the in vivo chemoresistance of NSCLC.
  • These findings suggest that other mechanisms may be more critical in conferring drug resistance in NSCLC.

Related Experiment Videos

Last Updated: Jun 22, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
12:33

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013