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Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
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Published on: August 9, 2019

Delayed fluorescence in photosynthesis.

Vasilij Goltsev1, Ivelina Zaharieva, Petko Chernev

  • 1Department of Biophysics and Radiobiology, Faculty of Biology, St. Kliment Ohridski University of Sofia, 8 Dragan Tzankov Boulevard, Sofia, Bulgaria. goltsev@biofac.uni-sofia.bg

Photosynthesis Research
|June 24, 2009
PubMed
Summary
This summary is machine-generated.

Plants emit light through chlorophyll fluorescence, a process revealing insights into Photosystem II (PS II) electron transport. Delayed fluorescence (DF) specifically probes redox reactions, offering a window into plant photosynthesis dynamics.

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Area of Science:

  • Plant Physiology
  • Photochemistry
  • Photosynthesis Research

Background:

  • Photosynthesis, while efficient, involves light emission from excited chlorophyll molecules.
  • Prompt fluorescence occurs before energy utilization, while delayed fluorescence (DF) arises from charge recombination.
  • DF intensity kinetics reflect electron transport on both donor and acceptor sides of Photosystem II (PS II).

Purpose of the Study:

  • To review historical and recent data on delayed fluorescence (DF) research.
  • To elucidate the relationship between DF and specific reactions within Photosystem II (PS II).
  • To discuss an experimental method for simultaneous DF and prompt fluorescence measurements.

Main Methods:

  • Recording DF decay after light excitation (single flash or continuous).
  • Measuring DF intensity during light adaptation (induction curves).
  • Simultaneous recording of prompt and delayed fluorescence induction and DF decay in the millisecond domain.

Main Results:

  • DF intensity is a polyphasic function of time post-illumination.
  • DF kinetics are directly linked to electron transport reactions in PS II.
  • The reviewed data highlight DF as a sensitive indicator of PS II function.

Conclusions:

  • Delayed fluorescence (DF) provides valuable information about electron transport in Photosystem II (PS II).
  • DF measurements offer a non-invasive method to study photosynthetic processes.
  • Advanced techniques allow simultaneous analysis of prompt and delayed fluorescence for comprehensive insights.