Related Experiment Video
Updated: Jun 22, 2026
Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis
Published on: September 26, 2022
[Immune function changes caused by contact dermatitis in mice after intracutaneous injection of trichloroethylene]
Yan Wang1, Baixiang Li, Shuying Gao
1Department of Toxicology, Public Health College, Harbin Medical University, Harbin 150086, China. wangyan782913@163.com
Objective:
To investigate the contact dermatitis of BALB/c mice exposed to Trichloroethylene (TCE) via back intradermal injection and observe the influence on immune function of BALB/c mice.
Methods:
BALB/c mice were exposed to TCE through intradermal injection. The organ coefficient of spleen and thymus were calculated. Lymphocytes viability and lymphocytes proliferation assay, Macrophage activation and NK cell activity assay were carried out; the content of TNF-alpha and IFN-gamma in the serum were measured.
Results:
The thymic organ coefficient of TCE group was significantly lower than that of the negative control group (P < 0.01). The lymphocytes viability and lymphocytes proliferation of TCE group were obviously higher than those of the negative control group (P < 0.01). Compared to negative control group, the response of macrophage activation and NK cell activity of the TCE group were enhanced markedly (P < 0.01). In TCE group the serous content of TNF-alpha was evidently higher than that of the negative control group (P < 0.01).
Conclusion:
Trichloroethylene as an allergen can cause contact dermatitis whose mechanism was supposed to be a delayed-type hypersensitivity.
