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Related Concept Videos

The Spindle Assembly Checkpoint

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

The Spindle Assembly Checkpoint

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

Anaphase A and B

Anaphase A and B

Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...

Anaphase A and B

Anaphase A and B

Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...

Forces Acting on Chromosomes

Forces Acting on Chromosomes

During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...

Forces Acting on Chromosomes

Forces Acting on Chromosomes

During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...

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Related Experiment Video

Updated: Jun 22, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Chromosome segregation: monopolin goes spindle.

Anton Khmelinskii¹, Elmar Schiebel

¹Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Allianz, Im Neuenheiemer Feld 282, 69117 Heidelberg, Germany.

Current Biology : CB

|June 25, 2009

Summary

The monopolin complex stabilizes the mitotic spindle during anaphase. This finding reveals a new role for the monopolin complex in cell division beyond its known function in meiosis.

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

The mitotic spindle is crucial for segregating sister chromatids during cell division.
The monopolin complex is primarily known for its role at kinetochores during meiosis.

Purpose of the Study:

To investigate the localization and function of the monopolin complex during mitosis.
To determine if the monopolin complex has a role in stabilizing the mitotic spindle at anaphase onset.

Main Methods:

Immunofluorescence microscopy to visualize monopolin complex localization.
Live-cell imaging to observe spindle dynamics.
Biochemical assays to assess spindle stability.

Main Results:

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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

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Related Experiment Videos

Last Updated: Jun 22, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

The monopolin complex localizes to the mitotic spindle during anaphase.

The presence of the monopolin complex stabilizes the anaphase spindle.

This stabilization is independent of its known meiotic function.

Conclusions:

The monopolin complex plays a novel role in mitotic spindle stabilization.
This discovery expands our understanding of cell division regulation.
Further research is warranted to elucidate the precise mechanisms involved.