Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epigenetic clocks as quantitative readouts of epigenetic drift.

Nature aging·2026
Same author

Statins May Not Reduce Reoperation Rates in Chronic Subdural Hematoma, Regardless of the Use of Antithrombotic Medication.

Journal of neurological surgery. Part A, Central European neurosurgery·2026
Same author

Correction: Jugel et al. Targeted Transposition of Minicircle DNA Using Single-Chain Antibody Conjugated Cyclodextrin-Modified Poly (Propylene Imine) Nanocarriers. <i>Cancers</i> 2022, <i>14</i>, 1925.

Cancers·2026
Same author

Fluctuating DNA methylation tracks cancer evolution at clinical scale.

Nature·2025
Same author

Molecular landscape and clinical correlates of olfactory groove meningiomas: a multi-institutional study.

Journal of neurosurgery·2025
Same author

Age-dependent effect of environmental enrichment on the neural 3D chromatin interactome.

Cell reports·2025

Related Experiment Video

Updated: Jun 22, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

A microarray-based DNA methylation study of glioblastoma multiforme.

Ramon Martinez1, Jose I Martin-Subero, Veit Rohde

  • 1Department of Neurosurgery, University of Goettingen, Goettingen WT-084071, Germany. ramon.martinez@med.uni-goettingen.de

Epigenetics
|June 25, 2009
PubMed
Summary

This study reveals genome-wide DNA methylation changes in glioblastoma multiforme (GBM), identifying specific genes and linking methylation patterns to patient survival and potential stem cell origins.

More Related Videos

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
05:45

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

Related Experiment Videos

Last Updated: Jun 22, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
05:45

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

Area of Science:

  • Oncology
  • Epigenetics
  • Genomics

Background:

  • Glioblastoma multiforme (GBM) is a lethal primary brain tumor.
  • Epigenetic alterations, including MGMT gene hypermethylation, are known in GBM.
  • A comprehensive genome-wide DNA methylation profile for GBM is lacking.

Purpose of the Study:

  • To perform a genome-wide DNA methylation analysis in GBM.
  • To identify novel hypermethylated and hypomethylated genes in GBM.
  • To correlate DNA methylation profiles with patient survival and GBM cellular origins.

Main Methods:

  • Analyzed DNA methylation levels of 1,505 CpG sites across 807 genes in 87 GBM samples using universal BeadArrays.
  • Utilized supervised cluster analyses to identify differentially methylated genes.
  • Investigated the biological pathways and prognostic implications of identified methylation patterns.

Main Results:

  • Identified 25 hypermethylated and 7 hypomethylated genes in over 20% of GBM cases.
  • HOXA11, CD81, and TNFRSF10A were among the most frequently hypermethylated genes.
  • Hypermethylated genes were enriched for Polycomb repressive complex 2 (PRC2) targets, suggesting stem cell origins.
  • MGMT methylation showed a favorable prognostic impact in patients treated with alkylating agents.
  • GATA6 promoter hypermethylation was associated with unfavorable patient survival.

Conclusions:

  • GBM exhibits widespread DNA methylation changes, including novel hypermethylated and hypomethylated genes.
  • The enrichment of PRC2 targets suggests GBM may arise from cells with stem cell-like properties.
  • Specific DNA methylation profiles, such as GATA6 hypermethylation, are significant prognostic markers for GBM patient survival.