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Related Experiment Video

Updated: Jun 22, 2026

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

High-resolution computed tomography with a compact soft x-ray microscope.

Michael Bertilson¹, Olov von Hofsten, Ulrich Vogt

¹Department of Applied Physics, Royal Institute of Technology/AlbaNova, Stockholm, Sweden. michael.bertilson@biox.kth.se

|June 25, 2009

Summary

This summary is machine-generated.

Researchers developed high-resolution soft x-ray tomography using a lab-based microscope, enabling 3D imaging of biological structures. This advance increases accessibility to sub-visible-microscopic resolution for scientific study.

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Area of Science:

Microscopy
Imaging Science
Biophysics

Background:

High-resolution soft x-ray microscopy offers sub-visible-microscopic 3D structural analysis of biological specimens.
Current techniques are limited to synchrotron-based facilities, restricting researcher access.

Purpose of the Study:

To demonstrate high-resolution soft x-ray tomography using a laboratory-based zone-plate microscope.
To overcome limitations of synchrotron-based access for advanced biological imaging.

Main Methods:

Utilized a laboratory zone-plate-based soft x-ray microscope.
Acquired a tilt series of 53 images of a diatom specimen.
Reconstructed the 3D tomogram using a filtered back projection algorithm.

Main Results:

Successfully generated a high-resolution soft x-ray tomogram from laboratory-based microscopy.
Achieved a tomogram resolution of 140 nm using a differential-phase-residual method.
Demonstrated the feasibility of compact systems for biological sample imaging.

Conclusions:

Laboratory-based soft x-ray tomography provides accessible high-resolution 3D imaging of biological samples.
Future improvements in cryo-fixation, source brightness, and objectives will enhance compact system resolution.