Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

UV–Vis Spectroscopy of Conjugated Systems

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π&VeryThinSpace;→&VeryThinSpace;π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Retinal OCT Parameters and Physiological Dynamics as an Effect of Anesthesia in C57BL/6J Mice.

Investigative ophthalmology & visual science·2026

Same author

Evolving landscape of glioblastoma research : A 75-year bibliometric study on survival, treatment and gender in authorship.

Wiener klinische Wochenschrift·2026

Same author

Blood flow analysis of retinal neovascularisations in a VLDLR mouse model using contrast-enhanced optical coherence tomography.

Biomedical optics express·2026

Same author

Ultrasensitive Saliva-Based Detection of Early Alzheimer's Disease Biomarkers via Nanoparticle-Enhanced Evanescent Scattering Microscopy.

ACS sensors·2026

Same author

Longitudinal investigation of spatial memory and retinal parameters in a 5xFAD model of Alzheimer's disease reveals differences dependent on genotype and sex.

Biomedical optics express·2026

Same author

The retinal nerve fibre layer thickness slope: a localised biomarker of the structure-function relationship in early glaucoma.

The British journal of ophthalmology·2025

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Full range complex spectral domain optical coherence tomography without additional phase shifters.

Bernhard Baumann, Michael Pircher, Erich Götzinger

|June 25, 2009

Summary

This summary is machine-generated.

A novel full range complex spectral domain optical coherence tomography (FRC SD-OCT) method eliminates the need for extra phase shifting devices. This innovation doubles the accessible depth range for imaging biological tissues, including the human anterior chamber in vivo.

More Related Videos

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

Related Experiment Videos

Last Updated: Jun 22, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

Area of Science:

Biomedical Optics
Medical Imaging Technology
Ophthalmology

Background:

Spectral domain optical coherence tomography (SD-OCT) is crucial for high-resolution imaging.
Conventional FRC SD-OCT methods require specialized phase modulation devices.
Limitations exist in extending the imaging depth range without complex setups.

Purpose of the Study:

To introduce a new FRC SD-OCT method that simplifies hardware requirements.
To enhance the accessible depth range for OCT imaging.
To validate the technique for in vivo biological tissue imaging.

Main Methods:

A novel FRC SD-OCT system was developed without additional phase shifting devices.
Phase modulation was achieved using the galvanometer scanner by displacing the probe beam.
A Hilbert transform-based algorithm processed modulated spectral data to retrieve complex-valued signals.

Main Results:

The new FRC SD-OCT method successfully introduced phase shifts via the galvanometer scanner.
Quantitative measurements demonstrated effective suppression of mirror images.
High-speed, full-range images of the human anterior chamber in vivo were acquired.

Conclusions:

The presented FRC SD-OCT method offers a simplified and effective approach to OCT imaging.
This technique doubles the accessible depth range, improving imaging capabilities.
The method is validated for high-speed, in vivo imaging of biological structures.