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Endothelin-1 in luteal tissue
S Usuki1, N Suzuki, H Matsumoto
1Department of Obstetrics and Gynecology, University of Tsukuba, Ibaraki, Japan.
Molecular and Cellular Endocrinology
|September 1, 1991
Summary
This study detected high concentrations of endothelin-1 (ET-1) in rat corpora lutea (CL). The findings suggest ET-1 is an intraovarian peptide with a potential physiological role in the ovary.
Area of Science:
- Reproductive biology
- Endocrinology
- Ovarian physiology
Background:
- Endothelin-1 (ET-1) is a potent vasoactive peptide.
- The presence and function of ET-1 within the corpus luteum (CL) remain largely uncharacterized.
- A sensitive sandwich-enzyme immunoassay (EIA) for ET-1 was recently developed.
Purpose of the Study:
- To immunologically and biologically detect endothelin-1 (ET-1) in rat corpora lutea (CL).
- To investigate the concentration of ET-1 in the CL during different stages of development.
- To confirm the identity of immunoreactive ET-1 in the CL.
Main Methods:
- Utilized a highly sensitive and specific sandwich-enzyme immunoassay (EIA) for ET-1 detection.
- Analyzed ovaries from superovulated rats (PMSG/hCG) and pseudopregnant rats (PSP).
- Employed reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with EIA to characterize immunoreactive ET-1 (ir-ET-1).
Main Results:
- High concentrations of immunoreactive ET-1 (ir-ET-1) were detected in the rat corpus luteum (CL).
- RP-HPLC-EIA analysis confirmed that ir-ET-1 is identical to synthetic ET-1.
- ir-ET-1 levels were significantly higher in CL at 7 days post-hCG or post-PSP compared to 4 days.
Conclusions:
- Endothelin-1 (ET-1) is present in high concentrations within the rat corpus luteum (CL).
- ET-1 is identified as a novel intraovarian peptide.
- The quantity of ET-1 in the CL appears to vary with the age of the CL, suggesting a potential physiological role in ovarian function.