Characterizing and tracking single colloidal particles with video holographic microscopy
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Updated: Jun 22, 2026
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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
Sang-Hyuk Lee1, Yohai Roichman, Gi-Ra Yi
1Department of Physics and Center for Soft Matter Research, New York University, New York, NY 10003, USA.
Optics Express
|June 25, 2009
Abstract:
We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and refractive index to within 1%, and simultaneously to measure its three-dimensional position with nanometer in-plane precision and 10 nanometer axial resolution.
