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Updated: Jun 22, 2026
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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
Published on: March 10, 2017
Design and implementation of high-throughput screening assays.
Ricardo Macarrón1, Robert P Hertzberg
1Molecular Discovery Research, GlaxoSmithKline, Collegeville, PA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 25, 2009
Summary
High-throughput screening (HTS) is essential for drug discovery. Careful assay design involving multiple scientific disciplines is critical for successful lead compound identification.
Area of Science:
- Drug discovery and development
- Assay development
- Bioinformatics
Background:
- High-throughput screening (HTS) is a cornerstone of modern drug discovery.
- Effective HTS requires a multidisciplinary approach involving biology, chemistry, engineering, and informatics.
- Numerous variables influence HTS success, from target selection to lead compound identification.
Purpose of the Study:
- To emphasize the critical importance of comprehensive HTS assay design and implementation.
- To highlight the impact of decision-making at each stage of the HTS process.
- To underscore the need for quality guidelines and pragmatic compromise in HTS.
Main Methods:
- N/A - This abstract describes a conceptual framework rather than a specific experiment.
- Cross-disciplinary collaboration in assay design.
- Systematic analysis of variables impacting HTS outcomes.
Main Results:
- N/A - This abstract describes a conceptual framework rather than a specific experiment.
- Decisions made during HTS assay development significantly influence success or failure.
- Pragmatism and compromise are necessary to balance opposing forces in HTS.
Conclusions:
- Successful HTS relies on meticulous planning and execution across diverse scientific fields.
- Establishing quality guidelines is crucial for reliable HTS results.
- Balancing scientific rigor with practical considerations is key to effective drug discovery via HTS.
