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Application of fluorescence polarization in HTS assays

Xinyi Huang1, Ann Aulabaugh

  • 1Chemical and Screening Sciences, Wyeth Research, Collegeville, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 25, 2009
PubMed

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...