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Chronic venous insufficiency is associated with elevated level of circulating microparticles

A Georgescu1, N Alexandru, D Popov

  • 1Institute of Cellular Biology and Pathology, 'Nicolae Simionescu', Bucharest, Romania. adriana.georgescu@icbp.ro

Journal of Thrombosis and Haemostasis : JTH
|June 26, 2009
PubMed
Abstract

Insights

Chronic venous insufficiency (CVI) involves elevated levels of endothelial (EMPs) and platelet microparticles (PMPs), which contribute to vein wall dysfunction. These microparticles play a key role in the altered venous response seen in CVI patients.

Area of Science:

  • Vascular Biology
  • Cellular Biology
  • Biomedical Science

Background:

  • Chronic venous insufficiency (CVI) impairs effective blood return from the legs to the heart.
  • Microparticles (MPs) are vesicles released by activated or apoptotic vascular and circulating cells.

Purpose of the Study:

  • To evaluate circulating endothelial microparticles (EMPs) and platelet microparticles (PMPs) in CVI patients.
  • To determine the role of EMPs and PMPs in human peripheral venule dysfunction.

Main Methods:

  • Explantation of human peripheral venules from CVI patients and controls.
  • Blood sample collection and isolation of circulating MPs.
  • Utilized flow cytometry, microscopy, myography, and western blotting.

Main Results:

  • CVI patients exhibited elevated circulating EMPs and PMPs compared to controls.
  • Observed structural venous wall modifications and altered reactivity associated with MP adherence.
  • Demonstrated impaired contractile and relaxation responses in CVI venules, with increased TF and P-Selectin expression.

Conclusions:

  • CVI is characterized by increased release of EMPs and PMPs.
  • These microparticles significantly contribute to the altered and dysfunctional venous wall response in CVI.

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