A phenotypic study of TFS1 mutants differentially altered in the inhibition of Ira2p or CPY
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Updated: Jun 22, 2026
Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
Published on: March 24, 2017
Aurélie Gombault1, Jonas Warringer, Robert Caesar
1Centre de Biophysique Moléculaire, Centre National de la Recherche Scientifique, UPR 4301, Affiliated to the University of Orléans and INSERM, Orléans, France.
Abstract:
The Saccharomyces cerevisiae protein Tfs1p is known as a dual protein. On the one hand, it inhibits the carboxypeptidase Y protease, and on the other, it inhibits Ira2p, a GTPase-activating protein of Ras. We managed to dissect precise areas of Tfs1p specifically involved in only one of those functions. Based on these data, specific Tfs1p point mutants affected in only one of these two functions were constructed. In order to obtain insights on the physiological role of these functions, systematic phenotypic tests were performed on strains expressing these specific Tfs1p mutants. The results obtained demonstrate that the inhibition of Ira2p by Tfs1p is the predominant function under the conditions tested.
