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Updated: Jun 22, 2026

09:06
Cryo-EM and Single-Particle Analysis with Scipion
Published on: May 29, 2021
Single-particle cryoEM reconstructions: meeting the challenge
1Unité de Virologie Structurale, Institut Pasteur, and Centre National de la Recherche Scientifique, Unité de Recherche Associée 3015, Paris, France. rey@pasteur.fr
Summary
No abstract available in PubMed .
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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...