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Cryo-EM and Single-Particle Analysis with Scipion
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Single-particle cryoEM reconstructions: meeting the challenge

Félix A Rey1

  • 1Unité de Virologie Structurale, Institut Pasteur, and Centre National de la Recherche Scientifique, Unité de Recherche Associée 3015, Paris, France. rey@pasteur.fr

Proceedings of the National Academy of Sciences of the United States of America
|June 26, 2009
PubMed
Summary

No abstract available in PubMed .

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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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