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Updated: Jun 22, 2026

High-throughput Detection Method for Influenza Virus
10:05

High-throughput Detection Method for Influenza Virus

Published on: February 4, 2012

Detection of novel (swine origin) H1N1 influenza A virus by quantitative real-time reverse transcription-PCR

Ruixue Wang, Zong-Mei Sheng, Jeffery K Taubenberger

    Journal of Clinical Microbiology
    |June 26, 2009
    PubMed
    Abstract

    No abstract available in PubMed .

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    Real Time RT-PCR

    Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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