Related Experiment Video
Updated: Jun 22, 2026
13:52
Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
Published on: March 19, 2014
Kindling the flame of integrin activation and function with kindlins
Edward F Plow1, Jun Qin, Tatiana Byzova
1Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, OH 44195, USA. plowe@ccf.org
Current Opinion in Hematology
|June 26, 2009
Summary
Kindlins are essential regulators of integrin function. Recent studies reveal distinct in-vivo functions for each kindlin family member, impacting development and cellular processes.
Area of Science:
- Molecular Biology
- Cell Biology
- Developmental Biology
Background:
- Kindlins are a family of proteins with high sequence homology and a conserved domain structure.
- Kindlins are closely associated with integrins, influencing integrin-dependent cellular functions.
- In vivo data on kindlin functions and mechanisms were previously limited.
Purpose of the Study:
- To review recent findings on the in vivo functions of the three kindlin family members.
- To elucidate the mechanisms by which kindlins regulate integrin function.
Main Methods:
- Review of recent studies on kindlin-deficient animal models.
- Analysis of human disease data linked to kindlin mutations.
- Integration of in vitro and in vivo data on kindlin-integrin interactions.
Main Results:
- Kindlin-1 deficiency causes skin and intestinal defects.
- Kindlin-2 deficiency leads to embryonic lethality due to cardiac defects.
- Kindlin-3 deficiency results in severe defects in blood cell function and is linked to a human bleeding disorder.
Conclusions:
- The three kindlin family members are crucial regulators of integrin function.
- Kindlin deficiencies lead to distinct and profound phenotypes in vivo.
- Kindlins play essential roles in cellular and organismal integrity.
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