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Relations between ameboid movement and membrane-controlled electrical currents.

R Nuccitelli, M M Poo, L F Jaffe

    The Journal of General Physiology
    |June 1, 1977
    PubMed
    Summary
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    Amebas exhibit electrical currents, including steady flows and pulses, crucial for movement and pseudopod formation. These ion currents, particularly calcium, play a vital role in ameba behavior and locomotion.

    Area of Science:

    • Cellular Electrophysiology
    • Single-celled Organism Biology

    Background:

    • Amebas exhibit complex behaviors like streaming and pseudopod formation.
    • Electrical activity in single-celled organisms is not fully understood.

    Purpose of the Study:

    • To investigate the patterns of electrical currents in amebas (Chaos chaos).
    • To understand the role of electrical currents in ameba movement and behavior.

    Main Methods:

    • Utilized an ultrasensitive extracellular vibrating probe to measure electrical currents.
    • Performed ion substitution studies to identify current carriers.

    Main Results:

    • Identified steady electrical currents entering the rear and leaving pseudopods, with density of 0.1-0.2 muA/cm2.

    Related Experiment Videos

  • Observed current pattern changes preceding streaming reversals, indicating tail-to-head transitions.
  • Characterized calcium-dependent current pulses (5-10s duration, 0.4 muA/cm2 peak density) associated with pseudopod initiation and retraction.

    • Conclusions:

    • Electrical currents are integral to ameba locomotion and shape changes.
    • Calcium ions are involved in carrying steady inward currents.
    • Stimulated and spontaneous electrical pulses are linked to key cellular events like pseudopod dynamics.