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Related Concept Videos

Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

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Measuring Replicative Life Span in the Budding Yeast
12:41

Measuring Replicative Life Span in the Budding Yeast

Published on: June 25, 2009

Measuring replicative life span in the budding yeast.

Kristan K Steffen1, Brian K Kennedy, Matt Kaeberlein

  • 1Department of Biochemistry, University of Washington, USA.

Journal of Visualized Experiments : Jove
|June 27, 2009
PubMed
Summary
This summary is machine-generated.

This study details a method for measuring replicative life span in budding yeast (Saccharomyces cerevisiae). This assay quantifies cell division to model aging in actively dividing cells, offering insights into conserved aging mechanisms.

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Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

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Area of Science:

  • Cellular Biology
  • Aging Research
  • Yeast Genetics

Background:

  • Aging is a universal biological process involving cellular deterioration.
  • Budding yeast (Saccharomyces cerevisiae) serves as a model organism for studying aging due to conserved longevity pathways.
  • Two primary yeast aging assays exist: chronological life span (post-mitotic cells) and replicative life span (mitotically active cells).

Purpose of the Study:

  • To present a generalized protocol for measuring the replicative life span of budding yeast.
  • To establish a standardized method for quantifying yeast cell division and senescence.

Main Methods:

  • Utilizes a manual micromanipulator with a fiber-optic needle for physical separation of mother and daughter cells.
  • Employs standard light microscopy (160X magnification) for cell differentiation.
  • Involves tracking the number of buds produced by individual mother cells over time.

Main Results:

  • The protocol enables precise measurement of replicative life span in Saccharomyces cerevisiae.
  • Researchers can differentiate mother and daughter cells using microscopy.
  • Typical yeast strains yield 20-30 daughter cells per mother cell.

Conclusions:

  • The described method provides a robust approach to studying aging in mitotically active cells.
  • This replicative life span assay is crucial for understanding conserved aging mechanisms across eukaryotes.
  • The protocol aids in quantifying age-associated phenotypes in yeast, facilitating further aging research.