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A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)
Published on: March 7, 2017
An experimental field protocol for investigating the postmortem interval using multidisciplinary indicators
K Schoenly1, K Griest, S Rhine
1Center for Medicolegal Research and Consultation, Albuquerque, NM.
Journal of Forensic Sciences
|September 1, 1991
Summary
This study introduces a new field protocol and apparatus for determining the postmortem interval. The method uses a multidisciplinary approach to analyze insect succession and decomposition patterns for forensic science.
Area of Science:
- Forensic Science
- Forensic Entomology
- Forensic Anthropology
- Forensic Pathology
Background:
- Accurate determination of the postmortem interval (PMI) is crucial in forensic investigations.
- Existing methods for PMI estimation can be limited by environmental factors and decompositional variability.
- A multidisciplinary approach is essential for comprehensive PMI analysis.
Purpose of the Study:
- To propose and describe an experimental field protocol for investigating the postmortem interval.
- To detail the design and function of specialized apparatus for data collection.
- To establish a standardized method for multidisciplinary teams in forensic investigations.
Main Methods:
- Utilized specially designed apparatus and both human and pig cadavers in an experimental field setting.
- Employed a dual-functioning insect trap to simultaneously capture arriving and emerging insect populations.
- Collected data on insect succession, soft tissue decomposition, skeletal disarticulation, and environmental influences.
Main Results:
- Presented results of trap performance tests conducted in an arid climate.
- Included preliminary arthropod data obtained from field-exposed pig carcasses.
- Demonstrated the protocol's capability to gather simultaneous data on multiple decompositional fronts.
Conclusions:
- The proposed protocol provides a robust framework for collecting critical baseline data for PMI determination.
- This multidisciplinary approach, integrating entomological, pathological, and anthropological data, enhances PMI accuracy.
- The developed apparatus and methods offer valuable tools for forensic investigators and law enforcement.

