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Microaerobic Fermentation of Bamboo Hydrolysate by Klebsiella pneumoniae03:14

Microaerobic Fermentation of Bamboo Hydrolysate by Klebsiella pneumoniae

Source: Wei, D., et al., Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock. J. Vis. Exp. (2017)This video demonstrates the microbial conversion of bamboo-derived sugars into 2,3-butanediol by culturing Klebsiella pneumoniae in a hydrolysate-based medium under microaerobic and mildly acidic...
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In Planta Gene Expression and Gene Editing in Moso Bamboo Leaves06:57

In Planta Gene Expression and Gene Editing in Moso Bamboo Leaves

In this study, a novel in planta gene expression and gene editing method mediated by Agrobacterium was developed in bamboo. This method greatly improved the efficiency of gene function validation in bamboo, which has significant implications for accelerating the process of bamboo...
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Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Bamboo powder was pretreated with NaOH and enzymatically hydrolyzed. The hydrolysate of bamboo was used as the feedstock for 2,3-butanediol, R-acetoin, 2-ketogluconic acid, and xylonic acid production by Klebsiella pneumoniae.
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Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues08:47

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

This manuscript describes the methods for induction and scoring of the experimental autoimmune encephalomyelitis (EAE) model, together with the assessment of immune cell distribution and mRNA cytokine levels in lymph nodes, spleen, blood and spinal cord using flow cytometry and quantitative PCR, respectively, at various disease...
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Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis09:20

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

We describe a method for implanting and maintaining glass windows over the exposed spinal cords of adult mice for studying experimental autoimmune encephalomyelitis. These windows provide chronic optical access to the spinal cord for monitoring cell dynamics at the subcellular level in live animals using two-photon...
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Induction of Experimental Autoimmune Hypophysitis in SJL Mice10:38

Induction of Experimental Autoimmune Hypophysitis in SJL Mice

This video shows how to induce autoimmune hypophysitis in SJL mice and how to assess its severity by...
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