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Updated: Jun 22, 2026

Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
Published on: May 31, 2019
A method for acetonitrile-free microcystin analysis and purification by high-performance liquid chromatography, using
Esme L Purdie1, Fiona M Young, Diana Menzel
1Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom. e.l.purdie@dundee.ac.uk
Abstract:
Cyanobacteria produce a wide range of potent toxins, including hepatotoxic microcystins. HPLC methods for microcystin analysis and purification almost invariably include acetonitrile in the elution gradient mobile phase. The recent, acute, global acetonitrile shortage requires that adequate methods are available for microcystin analysis and purification without the need for acetonitrile. Here we present a convenient methanol-based method for effective HPLC analysis and purification of the toxins, with full separation of a range of microcystin variants.
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