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Updated: Jan 21, 2026

A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
Published on: February 14, 2020
Theophylline attenuates hippocampal blood flow responses induced by tooth pulp stimulation in rats
Makoto Hasegawa1, Junichi Hada, Tetsuya Abe
1Department of Dentistry and Oral Surgery, Hyogo College of Medicine, 1-1, Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan. m-hase@hyo-med.ac.jp
Abstract:
In this study, we performed tests to determine whether tooth pulp stimulation (TPS) increases hippocampal blood flow (HBF), and if so, to investigate whether the increase in HBF is mediated via the activation of adenosine receptors. We measured HBF in urethane-anesthetized rats using laser Doppler flowmetry (LDF) and examined the effect of theophylline, a nonselective adenosine receptor antagonist, on TPS-induced HBF responses. TPS increased HBF, and its response was significantly attenuated by the intraperitoneal administration of theophylline (20 mg/kg). These results suggest that the HBF response induced by TPS may be, at least in part, produced through adenosine receptors.
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