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Related Concept Videos

Isochoric and Isobaric Processes01:21

Isochoric and Isobaric Processes

A thermodynamic process that occurs at constant volume is called an isochoric process. According to the first law of thermodynamics, heat supplied or removed from the system is partially utilized to perform work and change the internal energy of the system. However, in an isochoric process, the volume remains constant. Hence, the work done by the system is zero. Therefore, the exchange of heat changes the internal energy of the system only. 
Suppose 1000 g of water is heated from 40 degrees...
Isothermal Processes01:21

Isothermal Processes

A thermodynamic process that occurs at constant temperature is called an isothermal process. Heat slowly flows into the system or out of the system to maintain thermal equilibrium. Processes involving phase changes like water evaporation into steam or freezing water into ice at a constant temperature are examples of Isothermal Processes.
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Principles of Food Preservation01:27

Principles of Food Preservation

Food spoilage results from microbial growth, enzymatic activity, and environmental factors that gradually degrade the sensory, nutritional, and safety qualities of food. Preservation techniques aim to slow or halt these processes to extend shelf life and maintain product quality.A key concept in food microbiology is the microbial growth curve, which includes four phases: lag, exponential (log), stationary, and death. During the lag phase, bacteria adjust to their environment without significant...
Conservation of Mass in Fixed, Nondeforming Control Volume01:07

Conservation of Mass in Fixed, Nondeforming Control Volume

The principle of conservation of mass is fundamental in fluid dynamics and is crucial for analyzing flow within fixed control volumes, such as pipes or ducts. This principle states that the total mass within a control volume remains constant unless altered by the inflow or outflow of mass through the control surfaces. This results in a vital relationship for steady, incompressible flow where the mass entering a system equals the mass leaving it.
In the case of a sewer pipe, which can be modeled...
Methods of Controlling Food Spoilage01:26

Methods of Controlling Food Spoilage

Food spoilage is caused by microbial growth or by chemical and physical changes, all of which affect the taste, texture, and safety of food.Temperature-Based PreservationRefrigeration at 0–4 °C slows microbial growth and enzyme activity, making it ideal for short-term storage. However, certain spoilage organisms—such as psychrotrophs like Listeria monocytogenes—can still proliferate at these temperatures. Freezing below -18 °C further slows biological processes by forming ice crystals, which...
Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...

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Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion
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Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion

Published on: November 28, 2025

Isochoric preservation: a novel characterization method.

Jessica A Preciado1, Boris Rubinsky

  • 1Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA. preciado@engineeralum.berkeley.edu

Cryobiology
|June 30, 2009
PubMed
Summary
This summary is machine-generated.

Isochoric preservation minimizes pressure and lowers nucleation temperatures for antifreeze proteins compared to isobaric methods. This constant volume technique offers a promising approach for cryobiology applications.

Related Experiment Videos

Last Updated: Jun 22, 2026

Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion
08:15

Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion

Published on: November 28, 2025

Area of Science:

  • Cryobiology
  • Thermodynamics
  • Biophysics

Background:

  • Isochoric (constant volume) preservation offers an alternative to traditional cryopreservation.
  • It requires less cryoprotectant and is simpler to operate than conventional methods.

Purpose of the Study:

  • To validate that isochoric preservation minimizes pressure for a given temperature.
  • To examine the effect of isochoric conditions on freezing point nucleation in antifreeze protein solutions.
  • To generate pressure-temperature phase diagrams for cryoprotectant solutions.

Main Methods:

  • Collected pressure and temperature data using a specially designed pressure vessel.
  • Investigated freezing point nucleation in aqueous antifreeze protein solutions under isochoric conditions.
  • Generated pressure-temperature phase diagrams for various cryoprotectant solutions.

Main Results:

  • The isochoric pressure vessel accurately followed the pressure-temperature phase diagram of water.
  • Nucleation temperature of antifreeze protein was lower in an isochoric vessel compared to isobaric methods.
  • Nucleation temperature decreased with increasing cryoprotectant concentration under isochoric conditions.
  • Cryoprotectant phase diagrams showed similar pressure-temperature slopes, indicating consistent effects of isochoric freezing.

Conclusions:

  • Isochoric preservation effectively minimizes pressure and influences ice formation.
  • The method demonstrates potential for characterizing cryoprotectant thermodynamics.
  • Isochoric preservation shows significant promise as a cryopreservation technique in cryobiology.