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NGSP and IFCC-derived NGSP HbA1c can be used interchangeably
K Paisooksantivatana1, A Kongsomgan, K Khupulsup
1Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Thailand. rakpz@mahidol.ac.th
Diabetes Research and Clinical Practice
|June 30, 2009
Summary
The National Glycohemoglobin Standardization Program (NGSP) certified method and the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) calibrated method for HbA1c measurement show excellent comparability. These methods can be used interchangeably in clinical practice.
Area of Science:
- Clinical Chemistry
- Laboratory Medicine
- Diagnostic Assays
Background:
- Accurate hemoglobin A1c (HbA1c) measurement is crucial for diabetes mellitus management.
- Standardization of HbA1c assays is essential for consistent patient care and comparability of results across different laboratories.
- The National Glycohemoglobin Standardization Program (NGSP) and International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) provide calibration and certification frameworks.
Purpose of the Study:
- To evaluate the comparability between an NGSP-certified method and an IFCC-calibrated method for HbA1c determination.
- To assess the agreement and potential biases between these two widely used standardization approaches.
Main Methods:
- Hemoglobin A1c (HbA1c) was measured in 50 samples using two distinct analyzers.
- Comparability was statistically assessed using Deming regression analysis.
- Bias estimation and Friedman test were employed to further evaluate method agreement.
Main Results:
- A strong correlation was observed between the NGSP-certified method and the IFCC-calibrated method.
- Good agreement was demonstrated between the two measurement systems, indicating minimal systematic differences.
- Statistical analyses confirmed the interchangeability of results from both methods.
Conclusions:
- The NGSP-certified method and the IFCC-calibrated method for HbA1c provide comparable results.
- HbA1c values obtained using either the NGSP or IFCC standardization can be used interchangeably in clinical settings.
- This interchangeability supports the reliability of diabetes monitoring regardless of the specific assay standardization used.
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