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Updated: Jun 22, 2026
Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Development and validation of an improved liquid chromatography-mass spectrometry method for the determination of
C Bobin-Dubigeon1, M B Amiand, C Herrenknecht
1Department of Oncology Biology, CLCC René Gauducheau, Bd J Monod 44805 Nantes Saint Herblain Cedex, France. c-bobin@nantes.fnclcc.fr
Abstract:
An improved liquid chromatography-mass spectrometry method for the determination of pemetrexed in human plasma was developed and validated using a simple quadrupole LC-MS and a new SPE cartridge (Plexa Bond Elut). The analysis was achieved with a C18 analytical column using a mobile phase consisting of formic acid/acetonitrile and isocratic flow for 7 min. The linear ranges (r(2)>0.99) were found from 5 to 5000 ng/mL. The lower limit of detection was 2.5 ng/mL. Within-day and between-day precisions were less than 7.2% and inaccuracy did not exceed 2.8%. This new method is suitable to support pharmacokinetic studies and drug monitoring.
