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Related Concept Videos

Establishment and Characterization of Patient-derived Gastric Organoids from Biopsies of Benign Gastric Body and Antral Epithelium08:45

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Gastric patient-derived organoids find increasing use in research, yet formal protocols for generating human gastric organoids from single-cell digests with standardized seeding density are lacking. This protocol presents a detailed method for reliably creating gastric organoids from biopsy tissue obtained during upper...
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Laparoscopy-endoscopy cooperative surgery is appropriate for the treatment of gastric gastrointestinal stromal tumors less than 5 cm in size. It can achieve the respective advantages of endoscopic and laparoscopic surgeries while avoiding their...
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Multiplex in situ hybridization (ISH) was employed to simultaneously visualize the transcripts for two G protein-coupled receptors and one transcription factor in the entire vagal ganglionic complex of the adult mouse. This protocol could be used to generate accurate maps of the transcriptional profiles of vagal afferent...
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This protocol is for the performance of one-anastomosis gastric bypass (OAGB) on rat. The operator performs a long and tubular-stapled gastric pouch followed by hand-sewn anastomosis. For this model, the operator reproduces the same ratio between biliopancreatic and common limb in humans; therefore, the biliopancreatic limb measures 35 cm.
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Determination of gastric emptying with a non-invasive [13C]-octanoic acid breath test for tracking gastroparesis in female NOD LtJ...
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Numerous studies using gastric bypass rat models have been recently conducted to uncover the underlying physiological mechanisms of Roux-en-Y gastric bypass operations. This article aims to demonstrate and discuss the technical and experimental details of our published gastric bypass rat model to understand advantages and limitations of this experimental...
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Related Experiment Video

Updated: Feb 1, 2026

One-anastomosis Gastric Bypass OAGB in Rats
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Intestinal barrier function: molecular regulation and disease pathogenesis.

Katherine R Groschwitz1, Simon P Hogan

  • 1Division of Allergy and Immunology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA.

The Journal of Allergy and Clinical Immunology
|June 30, 2009
PubMed
Summary

The intestinal barrier, formed by epithelial cells, protects against toxins. Disrupted barrier function is linked to inflammatory diseases like celiac disease and inflammatory bowel disease.

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Related Experiment Videos

Last Updated: Feb 1, 2026

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Area of Science:

  • Gastroenterology
  • Immunology
  • Cell Biology

Background:

  • The intestinal epithelium forms a critical barrier, regulating nutrient absorption and defending against pathogens.
  • This barrier relies on protein networks, including desmosomes, adherens junctions, and tight junctions, that link cells and seal intercellular spaces.
  • Dysfunctional intestinal barrier is increasingly associated with autoimmune and inflammatory conditions.

Purpose of the Study:

  • To review the molecular composition and regulation of intestinal barrier function.
  • To explore the interplay between immunity (innate and adaptive) and the intestinal barrier.
  • To examine the impact of external factors on intestinal barrier integrity.

Main Methods:

  • Literature review and synthesis of current research.
  • Analysis of molecular mechanisms underlying intestinal barrier formation and maintenance.
  • Examination of clinical and experimental data linking barrier dysfunction to disease.

Main Results:

  • Detailed overview of the protein complexes (desmosomes, adherens junctions, tight junctions) crucial for barrier integrity.
  • Discussion of how immune cells and exogenous factors influence intestinal barrier function.
  • Evidence supporting intestinal barrier dysfunction as a key factor in inflammatory diseases.

Conclusions:

  • Intestinal barrier dysfunction is a significant contributor to the development of inflammatory diseases.
  • Understanding the molecular regulation of the intestinal barrier is vital for therapeutic strategies.
  • Further research into the interactions between the barrier, immunity, and external factors is warranted.