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Updated: Jun 22, 2026

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
Published on: September 1, 2015
The Effect of Beta Interferon on Dendritic Cells and Cytokine Synthesis by CD4+ T Cells
Saeid Abediankenari1, Davoud Shaker, Farshideh Abedian
1Department of Microbiology and Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. abedianlab@yahoo.co.uk
Background:
Dendritic cells (DC) are a key regulator of the immune response, and interferon-beta (IFN-beta) is considered an immunomodulatory molecule for DC.
Objective:
The purpose of this study was to evaluate the ability of IFN-beta treated DC to induce cytokine secretion by CD4+ T cells.
Methods:
Dendritic cells were generated from blood monocytes with granulocyte-monocyte colony-stimulating factor and interleukin-4 with or without IFN-beta. We analyzed the production of CD4+ T helper cytokines (IL-17, IFN-gamma and IL-10) in the supernatant of the dendritic cell-T cell co- cultures by ELISA. We also studied the effects of HLA-G and costimulatory molecules on immature and mature DC.
Results:
IFN-gamma and IL-17 decreased significantly in the presence of HLA-G-bearing DC compared to control cultures (p<0.05).
Conclusion:
Using the mixed leukocyte reaction, we found that DC treated with IFN-beta mediated the inhibition of T cell activation via cytokine production. We conclude that this is important for preventing overactivation of the immune system.
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