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Related Concept Videos

Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

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Related Experiment Video

Updated: Jun 22, 2026

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Surface Plasmon Coupled Phosphorescence (SPCP).

Michael J R Previte1, Kadir Aslan, Yongxia Zhang

  • 1Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard St., Baltimore, MD 21201.

Chemical Physics Letters
|June 30, 2009
PubMed
Summary
This summary is machine-generated.

Researchers observed surface plasmon coupled phosphorescence (SPCP) in platinum octaethylporphyrin (PtOEP) within PVC films. This directional emission offers new possibilities for studying weak phosphorescence signals in biological applications.

Related Experiment Videos

Last Updated: Jun 22, 2026

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Area of Science:

  • Optoelectronics
  • Materials Science
  • Biophysics

Background:

  • Phosphorescence is a key photophysical process.
  • Isotropic emission limits sensitivity in biological studies.
  • Surface plasmon resonance (SPR) can modify light emission.

Purpose of the Study:

  • To report the first observation of surface plasmon coupled phosphorescence (SPCP).
  • To investigate SPCP using platinum octaethylporphyrin (PtOEP) immobilized in polyvinyl chloride (PVC) films.
  • To explore the potential of SPCP for enhanced biological sensing.

Main Methods:

  • Immobilization of PtOEP in PVC films on 47 nm gold films.
  • Comparison of SPCP spectra and lifetimes with free-space phosphorescence.
  • Characterization of directional emission properties.

Main Results:

  • Successful observation and confirmation of SPCP for PtOEP/PVC/Au system.
  • Demonstration of directional emission distinct from isotropic phosphorescence.
  • Quantification of spectral and lifetime changes due to plasmon coupling.

Conclusions:

  • SPCP is achievable with PtOEP in PVC films on gold substrates.
  • The directional nature of SPCP enhances signal detection.
  • SPCP holds promise for sensitive detection in biological applications like protein dynamics and enzyme assays.