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Related Experiment Video

Updated: Jun 22, 2026

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

MS binding assays-with MALDI toward high throughput.

Georg Höfner¹, Dietrich Merkel, Klaus Theodor Wanner

¹Department Pharmazie-Zentrum für Pharmaforschung, Ludwig-Maximilians-Universität München, Butenandtstr. 7, 81377 München, Germany.

|June 30, 2009

Summary

A new mass spectrometry (MS) binding assay using MALDI-MS-MS (FlashQuant) offers a faster, high-throughput alternative to traditional methods. This novel technique accurately quantifies drug targets like mGAT1, improving screening efficiency.

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Area of Science:

Biochemistry
Analytical Chemistry
Pharmacology

Background:

Radioligand binding assays are standard for measuring drug target interactions.
Conventional mass spectrometry (MS) binding assays using LC-ESI-MS-MS have throughput limitations.
High-throughput screening methods are crucial for drug discovery.

Purpose of the Study:

To establish a novel, high-throughput MS binding assay using MALDI-MS-MS.
To validate the FlashQuant system as a substitute for radioligand binding assays.
To quantify the mGAT1 transporter and its marker NO 711.

Main Methods:

Development of a quantification method for NO 711 using [2H10]NO 711 as an internal standard via MALDI-MS-MS.
Implementation of MS binding assays for mGAT1 in a 96-well format.
Determination of affinity constants through saturation and competition experiments.

Main Results:

A robust quantification method for NO 711 was established, covering a concentration range from 208 pM to 16.7 nM.
Affinity constants obtained using FlashQuant assays closely matched those from LC-ESI-MS-MS based assays.
The MALDI-MS system demonstrated rapid quantification (seconds per sample).

Conclusions:

The FlashQuant system provides a high-throughput, accurate, and efficient alternative to conventional MS binding assays.
This novel technique is suitable for screening drug candidates targeting transporters like mGAT1.
The method significantly enhances throughput capacity for MS-based binding assays.