[A proteomic analysis of human dental pulp cells undergoing odontoblast differentiation]
Xi Wei1, Li-ping Wu, Jun-qi Ling
1Department of Cariology and Endodontology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.
Objective:
To analyze the difference in protein profiles between human dental pulp cells (DPC) and odontogenic differentiated DPC by using proteomic approach.
Methods:
Human DPC were induced to odontoblast differentiation and total proteins in the cell lysates before and after induction were prepared. Proteins spots were isolated by two-dimensional gel electrophoresis. DeCyder V6.0 software was applied to gel image analysis. Differential protein spots were identified by peptide mass fingerprinting technique.
Results:
Forty-six protein spots were determined to be differentially expressed with twenty identified protein spots. Expression changes of the identified proteins revealed the involvement of various regulation mechanisms in odontoblast differentiation, such as cell cycle, cellular energy regulation and signal transduction.
Conclusions:
The proteomic approach is a high throughput method to screen the candidate proteins involved in odontoblast differentiation of DPC.
