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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jun 22, 2026

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome
14:23

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Published on: March 7, 2014

Enhanced detection method for corneal protein identification using shotgun proteomics.

Mitchell L Meade1, Pavel Shiyanov, John J Schlager

  • 1Applied Biotechnology Branch, Biosciences and Protection Division, Wright-Patterson AFB, Ohio, USA. mitchell.meade.ctr@wpafb.af.mil

Proteome Science
|July 1, 2009
PubMed
Summary

This study enhances cornea proteomics by separating tissue layers for analysis. This method identifies significantly more proteins, including novel ones, offering a deeper understanding of corneal health and disease.

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Last Updated: Jun 22, 2026

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome
14:23

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Published on: March 7, 2014

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

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Published on: April 1, 2017

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10:12

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Published on: October 28, 2021

Area of Science:

  • Ophthalmology
  • Proteomics
  • Biochemistry

Background:

  • The cornea, a transparent tissue, refracts light and maintains vision.
  • Corneal injury can lead to vision impairment by disrupting its structure.
  • Understanding corneal protein signatures is crucial for diagnosing and treating eye conditions.

Purpose of the Study:

  • To develop a more comprehensive proteomic profile of the mammalian cornea.
  • To investigate the utility of layer-specific proteomics in identifying corneal proteins.

Main Methods:

  • Rabbit corneas were dissected to separate epithelial and endothelial layers.
  • Proteins from each layer were analyzed using liquid chromatography/mass spectrometry (LC-MS).
  • Shotgun proteomics was employed with a six-hour chromatographic gradient and ion-trap mass spectrometry.

Main Results:

  • Over 350 proteins were identified, with over 225 being novel to corneal proteomics.
  • Layer-specific analysis identified nearly all proteins found in whole cornea samples.
  • Unique proteomes were discovered in the epithelium and endothelium, including olfactomedin-like 3 in the endothelium.

Conclusions:

  • Simple dissection and advanced LC-MS techniques significantly improve corneal proteome identification.
  • This enhanced proteomic profiling provides a more detailed understanding of corneal tissue.
  • Further research is needed to elucidate the function of newly identified proteins like olfactomedin-3 in the cornea.